• This series of NIST traceable size standards provides an accurate and convenient method for calibrating or checking the performance of laser particle counters used in cleanrooms and other contamination monitoring applications. The products are suspensions of monodisperse polystyrene spheres (PSL) for use in airborne or liquid particle dispersion systems. They are available in a range of sizes from 0.1 to 100µm in diameter. The particles are prepared as low residue aqueous suspensions for minimal background interference, packaged in 15mL dropper-tipped vials. The particle diameters are calibrated by electron or optical microscopy. Each package includes a Certificate of Calibration and Traceability to the National Institute of Standards and Technology (NIST). In comparison with the well established 3000 and 4000 Series products described elsewhere, these product lines are precisely diluted for immediate use in laser particle counters with minimal time-consuming adjustments of concentration.
  • This product line is intended for researchers who are developing lateral flow diagnostic tests, and for other scientific investigations in diagnostics and biotechnology where labeling and detection of very low concentrations of substances is required. Microspheres are available with black, blue and red visual colors and four different bright fluorescent colors. Carboxylate modified surface functionalities are available. The diameter coefficient of variation (C.V.) of the microspheres is less than 5%, ensuring uniform migration through membranes. The microspheres are 0.4 or 0,85 micrometers nominal diameter, where the first is an optimal size for most latex-based lateral flow tests. They are prepared without detergents so they can be used immediately without pre-cleaning. Color-Rich particles are loaded with up to 25 % dye by weight, much more than other comparable products. This results in high intensity particles that give superior test sensitivity in qualitative lateral flow applications. The introduction of quantitative lateral flow tests has created a need for colored and fluorescent microspheres that are designed specifically for this new technology. In addition to our high intensity colored products, we offer the same microspheres dyed with bright fluorescent dyes.
  • This product line is intended for researchers who are developing lateral flow diagnostic tests, and for other scientific investigations in diagnostics and biotechnology where labeling and detection of very low concentrations of substances is required. Microspheres are available with black, blue and red visual colors and four different bright fluorescent colors. Carboxylate modified surface functionalities are available. The diameter coefficient of variation (C.V.) of the microspheres is less than 5%, ensuring uniform migration through membranes. The microspheres are 0.4 or 0,85 micrometers nominal diameter, where the first is an optimal size for most latex-based lateral flow tests. They are prepared without detergents so they can be used immediately without pre-cleaning. Color-Rich particles are loaded with up to 25 % dye by weight, much more than other comparable products. This results in high intensity particles that give superior test sensitivity in qualitative lateral flow applications. The introduction of quantitative lateral flow tests has created a need for colored and fluorescent microspheres that are designed specifically for this new technology. In addition to our high intensity colored products, we offer the same microspheres dyed with bright fluorescent dyes.
  • This product line is intended for researchers who are developing lateral flow diagnostic tests, and for other scientific investigations in diagnostics and biotechnology where labeling and detection of very low concentrations of substances is required. Microspheres are available with black, blue and red visual colors and four different bright fluorescent colors. Carboxylate modified surface functionalities are available. The diameter coefficient of variation (C.V.) of the microspheres is less than 5%, ensuring uniform migration through membranes. The microspheres are 0.4 or 0,85 micrometers nominal diameter, where the first is an optimal size for most latex-based lateral flow tests. They are prepared without detergents so they can be used immediately without pre-cleaning. Color-Rich particles are loaded with up to 25 % dye by weight, much more than other comparable products. This results in high intensity particles that give superior test sensitivity in qualitative lateral flow applications. The introduction of quantitative lateral flow tests has created a need for colored and fluorescent microspheres that are designed specifically for this new technology. In addition to our high intensity colored products, we offer the same microspheres dyed with bright fluorescent dyes.
  • This product line is intended for researchers who are developing lateral flow diagnostic tests, and for other scientific investigations in diagnostics and biotechnology where labeling and detection of very low concentrations of substances is required. Microspheres are available with black, blue and red visual colors and four different bright fluorescent colors. Carboxylate modified surface functionalities are available. The diameter coefficient of variation (C.V.) of the microspheres is less than 5%, ensuring uniform migration through membranes. The microspheres are 0.4 or 0,85 micrometers nominal diameter, where the first is an optimal size for most latex-based lateral flow tests. They are prepared without detergents so they can be used immediately without pre-cleaning. Color-Rich particles are loaded with up to 25 % dye by weight, much more than other comparable products. This results in high intensity particles that give superior test sensitivity in qualitative lateral flow applications. The introduction of quantitative lateral flow tests has created a need for colored and fluorescent microspheres that are designed specifically for this new technology. In addition to our high intensity colored products, we offer the same microspheres dyed with bright fluorescent dyes.
  • This product line is intended for researchers who are developing lateral flow diagnostic tests, and for other scientific investigations in diagnostics and biotechnology where labeling and detection of very low concentrations of substances is required. Microspheres are available with black, blue and red visual colors and four different bright fluorescent colors. Carboxylate modified surface functionalities are available. The diameter coefficient of variation (C.V.) of the microspheres is less than 5%, ensuring uniform migration through membranes. The microspheres are 0.4 or 0,85 micrometers nominal diameter, where the first is an optimal size for most latex-based lateral flow tests. They are prepared without detergents so they can be used immediately without pre-cleaning. Color-Rich particles are loaded with up to 25 % dye by weight, much more than other comparable products. This results in high intensity particles that give superior test sensitivity in qualitative lateral flow applications. The introduction of quantitative lateral flow tests has created a need for colored and fluorescent microspheres that are designed specifically for this new technology. In addition to our high intensity colored products, we offer the same microspheres dyed with bright fluorescent dyes.
  • This product line is intended for researchers who are developing lateral flow diagnostic tests, and for other scientific investigations in diagnostics and biotechnology where labeling and detection of very low concentrations of substances is required. Microspheres are available with black, blue and red visual colors and four different bright fluorescent colors. Carboxylate modified surface functionalities are available. The diameter coefficient of variation (C.V.) of the microspheres is less than 5%, ensuring uniform migration through membranes. The microspheres are 0.4 or 0,85 micrometers nominal diameter, where the first is an optimal size for most latex-based lateral flow tests. They are prepared without detergents so they can be used immediately without pre-cleaning. Color-Rich particles are loaded with up to 25 % dye by weight, much more than other comparable products. This results in high intensity particles that give superior test sensitivity in qualitative lateral flow applications. The introduction of quantitative lateral flow tests has created a need for colored and fluorescent microspheres that are designed specifically for this new technology. In addition to our high intensity colored products, we offer the same microspheres dyed with bright fluorescent dyes.
  • Fluoro-Max™
  • Fluoro-Max™
  • Fluoro-Max™
  • Fluoro-Max™
  • Fluoro-Max™
  • Fluoro-Max™
  • Achieve maximum reactivity in many diagnostic applications with Thermo Scientific™ Opti-Bind™ Polystyrene Sulfate Particles for use in turbidimetric/nephelometric assays, slide agglutination assays, chemiluminescent assays and in clinical diagnostics and molecular biology applications. Particles can be used direct from the bottle, without any pre-cleaning. Our production does not utilize common surfactants (like SDS, Tween™ 20, Triton™ X-100) that can interfere with protein binding to particle surfaces. Polystyrene surfaces are very hydrophobic and adsorb proteins almost instantaneously. Thermo’s proprietary anionic surfactant does not interfere with the binding of proteins or cause proteins to desorb from microparticle surfaces. • Available in a wide range of diameters (from 0.1 μm to 2.5 μm) • No pre-washing • Tight size distribution—CV<2% • Optimized colloidal stability
  • Achieve maximum reactivity in many diagnostic applications with Thermo Scientific™ Opti-Bind™ Polystyrene Sulfate Particles for use in turbidimetric/nephelometric assays, slide agglutination assays, chemiluminescent assays and in clinical diagnostics and molecular biology applications. Particles can be used direct from the bottle, without any pre-cleaning. Our production does not utilize common surfactants (like SDS, Tween™ 20, Triton™ X-100) that can interfere with protein binding to particle surfaces. Polystyrene surfaces are very hydrophobic and adsorb proteins almost instantaneously. Thermo’s proprietary anionic surfactant does not interfere with the binding of proteins or cause proteins to desorb from microparticle surfaces. • Available in a wide range of diameters (from 0.1 μm to 2.5 μm) • No pre-washing • Tight size distribution—CV<2% • Optimized colloidal stability
  • carboxylate particles Control sensitivity, specificity and stability with various acid content available within Thermo Scientific™ Opti-Link™ Carboxylate-Modified Particles. Particles contain carboxylic acid groups for covalent coupling and can be used directly from the bottle in a variety of applications. • Parking area combines surface area and acid content to give you the surface acid distribution that is useful for assay optimization • Surface area per gram of a particle varies inversely with the particle diameter • Acid content refers to the titrated milliequivalents per gram (mEq/g) and is weight-based • For most applications, particles can be used directly from the bottle without any pre-cleaning • Proprietary anionic surfactant does not interfere with binding of proteins nor cause proteins to desorb from particle surfaces or cause proteins to desorb from particle surfaces • Various surface acid concentrations available to help optimize reagent development efforts • Particles have been used in and are equally successful in molecular biology and research applications Parking Area Properties • Low Acid Content—60–100 Parking Area—Hydrophobic surface, more colloidally stable than plain polystyrene • Medium Acid Content—35–59 Parking Area—Hydrophobic and hydrophilic areas; good colloidal stability and good covalent coupling • High Acid Content—10–34 Parking Area—Hydrophilic surface; excellent colloidal stability; excellent covalent coupling When using Opti-Link particles, ask about our Microparticle Reagent Optimization manual, which outlines our reagent development strategy. This manual includes validated binding, coupling and particle reagent optimization protocols. Power-Bind Sterptavidin Onderstaande tekst volledig overgenomen van Thermo website Bind a variety of biotinylated ligands, including DNA/RNA, oligos, antibodies and proteins with Thermo Scientific™ Power-Bind Streptavidin Coated Particles. Particles have very high and stable biotin binding capacities and address the concerns of diagnostic test kit manufacturers and nucleic acid researchers. The microparticles reduce serum matrix effects and improve assay accuracy, resulting in low, non-specific binding of serum proteins and other interfering substances. The use of microparticles as a solid phase support in various immunoassays and affinity purifications has been known for some time; however, achieving high activity and stable binding of solid phase ligands has been a major difficulty. Biotinylated compounds become stably bound to Power-Bind Streptavidin Coated particles after simple incubation in buffer due to the high affinity of the biotin-streptavidin interaction. The particles also function as a spacer, which improves the specific activity of the bound ligand. Monodisperse nonmagnetic particle suspensions with streptavidin covalently bound to the surface in a highly active form Features: • Made from 0.3μm or 0.8μm Thermo Scientific™ OptiLink™ Carboxylate-Modified particles • High biotin-binding capacity • Long shelf life • Simplifies the process of binding ligands to particles • Combines the advantages of high surface area and easy, high affinity, high specific activity binding • Excellent for chemiluminescent assays, biotinylated ligand capture, and for molecular biology, immunoassay, or sample prep applications • • Benefits: • Dissociation constant (Kd 10-15 molar), easy one-step binding protocols for biotinylated ligands • High activity of surface bound ligands to easily solve difficult coupling problems • Simple aqueous biotinylation reactions • Low nonspecific interactions (blocked microparticle surface) • Highly mobile particles for membrane-based applications • Can be used in EIA formats with biotin/enzyme detection systems • Beneficial spacer effect of SA molecule • A choice of 0.3μm or 0.8μm diameters for different application requirements Recommended for: • Universal nonmagnetic bead for coupling unique ligands. • Clinical diagnostics and molecular biology applications.
  • carboxylate particles Control sensitivity, specificity and stability with various acid content available within Thermo Scientific™ Opti-Link™ Carboxylate-Modified Particles. Particles contain carboxylic acid groups for covalent coupling and can be used directly from the bottle in a variety of applications. • Parking area combines surface area and acid content to give you the surface acid distribution that is useful for assay optimization • Surface area per gram of a particle varies inversely with the particle diameter • Acid content refers to the titrated milliequivalents per gram (mEq/g) and is weight-based • For most applications, particles can be used directly from the bottle without any pre-cleaning • Proprietary anionic surfactant does not interfere with binding of proteins nor cause proteins to desorb from particle surfaces or cause proteins to desorb from particle surfaces • Various surface acid concentrations available to help optimize reagent development efforts • Particles have been used in and are equally successful in molecular biology and research applications Parking Area Properties • Low Acid Content—60–100 Parking Area—Hydrophobic surface, more colloidally stable than plain polystyrene • Medium Acid Content—35–59 Parking Area—Hydrophobic and hydrophilic areas; good colloidal stability and good covalent coupling • High Acid Content—10–34 Parking Area—Hydrophilic surface; excellent colloidal stability; excellent covalent coupling When using Opti-Link particles, ask about our Microparticle Reagent Optimization manual, which outlines our reagent development strategy. This manual includes validated binding, coupling and particle reagent optimization protocols. Power-Bind Sterptavidin Onderstaande tekst volledig overgenomen van Thermo website Bind a variety of biotinylated ligands, including DNA/RNA, oligos, antibodies and proteins with Thermo Scientific™ Power-Bind Streptavidin Coated Particles. Particles have very high and stable biotin binding capacities and address the concerns of diagnostic test kit manufacturers and nucleic acid researchers. The microparticles reduce serum matrix effects and improve assay accuracy, resulting in low, non-specific binding of serum proteins and other interfering substances. The use of microparticles as a solid phase support in various immunoassays and affinity purifications has been known for some time; however, achieving high activity and stable binding of solid phase ligands has been a major difficulty. Biotinylated compounds become stably bound to Power-Bind Streptavidin Coated particles after simple incubation in buffer due to the high affinity of the biotin-streptavidin interaction. The particles also function as a spacer, which improves the specific activity of the bound ligand. Monodisperse nonmagnetic particle suspensions with streptavidin covalently bound to the surface in a highly active form Features: • Made from 0.3μm or 0.8μm Thermo Scientific™ OptiLink™ Carboxylate-Modified particles • High biotin-binding capacity • Long shelf life • Simplifies the process of binding ligands to particles • Combines the advantages of high surface area and easy, high affinity, high specific activity binding • Excellent for chemiluminescent assays, biotinylated ligand capture, and for molecular biology, immunoassay, or sample prep applications • • Benefits: • Dissociation constant (Kd 10-15 molar), easy one-step binding protocols for biotinylated ligands • High activity of surface bound ligands to easily solve difficult coupling problems • Simple aqueous biotinylation reactions • Low nonspecific interactions (blocked microparticle surface) • Highly mobile particles for membrane-based applications • Can be used in EIA formats with biotin/enzyme detection systems • Beneficial spacer effect of SA molecule • A choice of 0.3μm or 0.8μm diameters for different application requirements Recommended for: • Universal nonmagnetic bead for coupling unique ligands. • Clinical diagnostics and molecular biology applications.
  • carboxylate particles Control sensitivity, specificity and stability with various acid content available within Thermo Scientific™ Opti-Link™ Carboxylate-Modified Particles. Particles contain carboxylic acid groups for covalent coupling and can be used directly from the bottle in a variety of applications. • Parking area combines surface area and acid content to give you the surface acid distribution that is useful for assay optimization • Surface area per gram of a particle varies inversely with the particle diameter • Acid content refers to the titrated milliequivalents per gram (mEq/g) and is weight-based • For most applications, particles can be used directly from the bottle without any pre-cleaning • Proprietary anionic surfactant does not interfere with binding of proteins nor cause proteins to desorb from particle surfaces or cause proteins to desorb from particle surfaces • Various surface acid concentrations available to help optimize reagent development efforts • Particles have been used in and are equally successful in molecular biology and research applications Parking Area Properties • Low Acid Content—60–100 Parking Area—Hydrophobic surface, more colloidally stable than plain polystyrene • Medium Acid Content—35–59 Parking Area—Hydrophobic and hydrophilic areas; good colloidal stability and good covalent coupling • High Acid Content—10–34 Parking Area—Hydrophilic surface; excellent colloidal stability; excellent covalent coupling When using Opti-Link particles, ask about our Microparticle Reagent Optimization manual, which outlines our reagent development strategy. This manual includes validated binding, coupling and particle reagent optimization protocols. Power-Bind Sterptavidin Onderstaande tekst volledig overgenomen van Thermo website Bind a variety of biotinylated ligands, including DNA/RNA, oligos, antibodies and proteins with Thermo Scientific™ Power-Bind Streptavidin Coated Particles. Particles have very high and stable biotin binding capacities and address the concerns of diagnostic test kit manufacturers and nucleic acid researchers. The microparticles reduce serum matrix effects and improve assay accuracy, resulting in low, non-specific binding of serum proteins and other interfering substances. The use of microparticles as a solid phase support in various immunoassays and affinity purifications has been known for some time; however, achieving high activity and stable binding of solid phase ligands has been a major difficulty. Biotinylated compounds become stably bound to Power-Bind Streptavidin Coated particles after simple incubation in buffer due to the high affinity of the biotin-streptavidin interaction. The particles also function as a spacer, which improves the specific activity of the bound ligand. Monodisperse nonmagnetic particle suspensions with streptavidin covalently bound to the surface in a highly active form Features: • Made from 0.3μm or 0.8μm Thermo Scientific™ OptiLink™ Carboxylate-Modified particles • High biotin-binding capacity • Long shelf life • Simplifies the process of binding ligands to particles • Combines the advantages of high surface area and easy, high affinity, high specific activity binding • Excellent for chemiluminescent assays, biotinylated ligand capture, and for molecular biology, immunoassay, or sample prep applications • • Benefits: • Dissociation constant (Kd 10-15 molar), easy one-step binding protocols for biotinylated ligands • High activity of surface bound ligands to easily solve difficult coupling problems • Simple aqueous biotinylation reactions • Low nonspecific interactions (blocked microparticle surface) • Highly mobile particles for membrane-based applications • Can be used in EIA formats with biotin/enzyme detection systems • Beneficial spacer effect of SA molecule • A choice of 0.3μm or 0.8μm diameters for different application requirements Recommended for: • Universal nonmagnetic bead for coupling unique ligands. • Clinical diagnostics and molecular biology applications.
  • carboxylate particles Control sensitivity, specificity and stability with various acid content available within Thermo Scientific™ Opti-Link™ Carboxylate-Modified Particles. Particles contain carboxylic acid groups for covalent coupling and can be used directly from the bottle in a variety of applications. • Parking area combines surface area and acid content to give you the surface acid distribution that is useful for assay optimization • Surface area per gram of a particle varies inversely with the particle diameter • Acid content refers to the titrated milliequivalents per gram (mEq/g) and is weight-based • For most applications, particles can be used directly from the bottle without any pre-cleaning • Proprietary anionic surfactant does not interfere with binding of proteins nor cause proteins to desorb from particle surfaces or cause proteins to desorb from particle surfaces • Various surface acid concentrations available to help optimize reagent development efforts • Particles have been used in and are equally successful in molecular biology and research applications Parking Area Properties • Low Acid Content—60–100 Parking Area—Hydrophobic surface, more colloidally stable than plain polystyrene • Medium Acid Content—35–59 Parking Area—Hydrophobic and hydrophilic areas; good colloidal stability and good covalent coupling • High Acid Content—10–34 Parking Area—Hydrophilic surface; excellent colloidal stability; excellent covalent coupling When using Opti-Link particles, ask about our Microparticle Reagent Optimization manual, which outlines our reagent development strategy. This manual includes validated binding, coupling and particle reagent optimization protocols. Power-Bind Sterptavidin Onderstaande tekst volledig overgenomen van Thermo website Bind a variety of biotinylated ligands, including DNA/RNA, oligos, antibodies and proteins with Thermo Scientific™ Power-Bind Streptavidin Coated Particles. Particles have very high and stable biotin binding capacities and address the concerns of diagnostic test kit manufacturers and nucleic acid researchers. The microparticles reduce serum matrix effects and improve assay accuracy, resulting in low, non-specific binding of serum proteins and other interfering substances. The use of microparticles as a solid phase support in various immunoassays and affinity purifications has been known for some time; however, achieving high activity and stable binding of solid phase ligands has been a major difficulty. Biotinylated compounds become stably bound to Power-Bind Streptavidin Coated particles after simple incubation in buffer due to the high affinity of the biotin-streptavidin interaction. The particles also function as a spacer, which improves the specific activity of the bound ligand. Monodisperse nonmagnetic particle suspensions with streptavidin covalently bound to the surface in a highly active form Features: • Made from 0.3μm or 0.8μm Thermo Scientific™ OptiLink™ Carboxylate-Modified particles • High biotin-binding capacity • Long shelf life • Simplifies the process of binding ligands to particles • Combines the advantages of high surface area and easy, high affinity, high specific activity binding • Excellent for chemiluminescent assays, biotinylated ligand capture, and for molecular biology, immunoassay, or sample prep applications • • Benefits: • Dissociation constant (Kd 10-15 molar), easy one-step binding protocols for biotinylated ligands • High activity of surface bound ligands to easily solve difficult coupling problems • Simple aqueous biotinylation reactions • Low nonspecific interactions (blocked microparticle surface) • Highly mobile particles for membrane-based applications • Can be used in EIA formats with biotin/enzyme detection systems • Beneficial spacer effect of SA molecule • A choice of 0.3μm or 0.8μm diameters for different application requirements Recommended for: • Universal nonmagnetic bead for coupling unique ligands. • Clinical diagnostics and molecular biology applications.
  • carboxylate particles Control sensitivity, specificity and stability with various acid content available within Thermo Scientific™ Opti-Link™ Carboxylate-Modified Particles. Particles contain carboxylic acid groups for covalent coupling and can be used directly from the bottle in a variety of applications. • Parking area combines surface area and acid content to give you the surface acid distribution that is useful for assay optimization • Surface area per gram of a particle varies inversely with the particle diameter • Acid content refers to the titrated milliequivalents per gram (mEq/g) and is weight-based • For most applications, particles can be used directly from the bottle without any pre-cleaning • Proprietary anionic surfactant does not interfere with binding of proteins nor cause proteins to desorb from particle surfaces or cause proteins to desorb from particle surfaces • Various surface acid concentrations available to help optimize reagent development efforts • Particles have been used in and are equally successful in molecular biology and research applications Parking Area Properties • Low Acid Content—60–100 Parking Area—Hydrophobic surface, more colloidally stable than plain polystyrene • Medium Acid Content—35–59 Parking Area—Hydrophobic and hydrophilic areas; good colloidal stability and good covalent coupling • High Acid Content—10–34 Parking Area—Hydrophilic surface; excellent colloidal stability; excellent covalent coupling When using Opti-Link particles, ask about our Microparticle Reagent Optimization manual, which outlines our reagent development strategy. This manual includes validated binding, coupling and particle reagent optimization protocols. Power-Bind Sterptavidin Onderstaande tekst volledig overgenomen van Thermo website Bind a variety of biotinylated ligands, including DNA/RNA, oligos, antibodies and proteins with Thermo Scientific™ Power-Bind Streptavidin Coated Particles. Particles have very high and stable biotin binding capacities and address the concerns of diagnostic test kit manufacturers and nucleic acid researchers. The microparticles reduce serum matrix effects and improve assay accuracy, resulting in low, non-specific binding of serum proteins and other interfering substances. The use of microparticles as a solid phase support in various immunoassays and affinity purifications has been known for some time; however, achieving high activity and stable binding of solid phase ligands has been a major difficulty. Biotinylated compounds become stably bound to Power-Bind Streptavidin Coated particles after simple incubation in buffer due to the high affinity of the biotin-streptavidin interaction. The particles also function as a spacer, which improves the specific activity of the bound ligand. Monodisperse nonmagnetic particle suspensions with streptavidin covalently bound to the surface in a highly active form Features: • Made from 0.3μm or 0.8μm Thermo Scientific™ OptiLink™ Carboxylate-Modified particles • High biotin-binding capacity • Long shelf life • Simplifies the process of binding ligands to particles • Combines the advantages of high surface area and easy, high affinity, high specific activity binding • Excellent for chemiluminescent assays, biotinylated ligand capture, and for molecular biology, immunoassay, or sample prep applications • • Benefits: • Dissociation constant (Kd 10-15 molar), easy one-step binding protocols for biotinylated ligands • High activity of surface bound ligands to easily solve difficult coupling problems • Simple aqueous biotinylation reactions • Low nonspecific interactions (blocked microparticle surface) • Highly mobile particles for membrane-based applications • Can be used in EIA formats with biotin/enzyme detection systems • Beneficial spacer effect of SA molecule • A choice of 0.3μm or 0.8μm diameters for different application requirements Recommended for: • Universal nonmagnetic bead for coupling unique ligands. • Clinical diagnostics and molecular biology applications.
  • carboxylate particles Control sensitivity, specificity and stability with various acid content available within Thermo Scientific™ Opti-Link™ Carboxylate-Modified Particles. Particles contain carboxylic acid groups for covalent coupling and can be used directly from the bottle in a variety of applications. • Parking area combines surface area and acid content to give you the surface acid distribution that is useful for assay optimization • Surface area per gram of a particle varies inversely with the particle diameter • Acid content refers to the titrated milliequivalents per gram (mEq/g) and is weight-based • For most applications, particles can be used directly from the bottle without any pre-cleaning • Proprietary anionic surfactant does not interfere with binding of proteins nor cause proteins to desorb from particle surfaces or cause proteins to desorb from particle surfaces • Various surface acid concentrations available to help optimize reagent development efforts • Particles have been used in and are equally successful in molecular biology and research applications Parking Area Properties • Low Acid Content—60–100 Parking Area—Hydrophobic surface, more colloidally stable than plain polystyrene • Medium Acid Content—35–59 Parking Area—Hydrophobic and hydrophilic areas; good colloidal stability and good covalent coupling • High Acid Content—10–34 Parking Area—Hydrophilic surface; excellent colloidal stability; excellent covalent coupling When using Opti-Link particles, ask about our Microparticle Reagent Optimization manual, which outlines our reagent development strategy. This manual includes validated binding, coupling and particle reagent optimization protocols. Power-Bind Sterptavidin Onderstaande tekst volledig overgenomen van Thermo website Bind a variety of biotinylated ligands, including DNA/RNA, oligos, antibodies and proteins with Thermo Scientific™ Power-Bind Streptavidin Coated Particles. Particles have very high and stable biotin binding capacities and address the concerns of diagnostic test kit manufacturers and nucleic acid researchers. The microparticles reduce serum matrix effects and improve assay accuracy, resulting in low, non-specific binding of serum proteins and other interfering substances. The use of microparticles as a solid phase support in various immunoassays and affinity purifications has been known for some time; however, achieving high activity and stable binding of solid phase ligands has been a major difficulty. Biotinylated compounds become stably bound to Power-Bind Streptavidin Coated particles after simple incubation in buffer due to the high affinity of the biotin-streptavidin interaction. The particles also function as a spacer, which improves the specific activity of the bound ligand. Monodisperse nonmagnetic particle suspensions with streptavidin covalently bound to the surface in a highly active form Features: • Made from 0.3μm or 0.8μm Thermo Scientific™ OptiLink™ Carboxylate-Modified particles • High biotin-binding capacity • Long shelf life • Simplifies the process of binding ligands to particles • Combines the advantages of high surface area and easy, high affinity, high specific activity binding • Excellent for chemiluminescent assays, biotinylated ligand capture, and for molecular biology, immunoassay, or sample prep applications • • Benefits: • Dissociation constant (Kd 10-15 molar), easy one-step binding protocols for biotinylated ligands • High activity of surface bound ligands to easily solve difficult coupling problems • Simple aqueous biotinylation reactions • Low nonspecific interactions (blocked microparticle surface) • Highly mobile particles for membrane-based applications • Can be used in EIA formats with biotin/enzyme detection systems • Beneficial spacer effect of SA molecule • A choice of 0.3μm or 0.8μm diameters for different application requirements Recommended for: • Universal nonmagnetic bead for coupling unique ligands. • Clinical diagnostics and molecular biology applications.
Go to Top