Color-Rich™

1.00

This product line is intended for researchers who are developing lateral flow diagnostic tests, and for other scientific investigations in diagnostics and biotechnology where labeling and detection of very low concentrations of substances is required. Microspheres are available with black, blue and red visual colors and four different bright fluorescent colors. Carboxylate modified surface functionalities are available. The diameter coefficient of variation (C.V.) of the microspheres is less than 5%, ensuring uniform migration through membranes. The microspheres are 0.4 or 0,85 micrometers nominal diameter, where the first is an optimal size for most latex-based lateral flow tests. They are prepared without detergents so they can be used immediately without pre-cleaning.
Color-Rich particles are loaded with up to 25 % dye by weight, much more than other comparable products. This results in high intensity particles that give superior test sensitivity in qualitative lateral flow applications. The introduction of quantitative lateral flow tests has created a need for colored and fluorescent microspheres that are designed specifically for this new technology. In addition to our high intensity colored products, we offer the same microspheres dyed with bright fluorescent dyes.

SKU: 4b619441bd80 Category:

Description

The purpose of a coloured or fluorescently dyed particle is its visibility with the naked eye or by a detector. By adding a dye to the particle it reduces signal to noise due to the contrast between the particle and its environment. Applications for these particles are countless. By modifying the surface of the particles, antibodies or proteins can be conjugated to their surfaces, making them ideally suited for diagnostic assay development, for instance for lateral flow assay development. We offer coloured and fluorescent dyed microspheres from Thermo Scientific, VDo Biotech and Applied Microspheres.

This product line is intended for researchers who are developing lateral flow diagnostic tests, and for other scientific investigations in diagnostics and biotechnology where labeling and detection of very low concentrations of substances is required. Microspheres are available with black, blue and red visual colors and four different bright fluorescent colors. Carboxylate modified surface functionalities are available. The diameter coefficient of variation (C.V.) of the microspheres is less than 5%, ensuring uniform migration through membranes. The microspheres are 0.4 or 0,85 micrometers nominal diameter, where the first is an optimal size for most latex-based lateral flow tests. They are prepared without detergents so they can be used immediately without pre-cleaning.
Color-Rich particles are loaded with up to 25 % dye by weight, much more than other comparable products. This results in high intensity particles that give superior test sensitivity in qualitative lateral flow applications. The introduction of quantitative lateral flow tests has created a need for colored and fluorescent microspheres that are designed specifically for this new technology. In addition to our high intensity colored products, we offer the same microspheres dyed with bright fluorescent dyes.

Polymer microspheres are a major component in commercially available diagnostic tests. To facilitate binding proteins or antibodies to their surfaces, different surface chemistries are available, suitable for all commonly used conjugation protocols. Dyed microspheres are available for visual detection methods and fluorescently dyed microspheres for devices using fluorescence as a method of detection.

To identify antibodies in a patient serum, the antigen of interest is attached to latex beads. When mixed with patient serum, the antibodies will bind the antigen, cross-linking the latex beads and causing the beads to agglutinate indirectly. This indicates the presence of the antibody of interest. This technique is most often used when looking for IgM antibodies, because their structure provides maximum cross-linking. One widely used example of this assay is a test for rheumatoid factor (RF) to confirm a diagnosis of rheumatoid arthritis. RF shows the presence of IgM antibodies that bind to the patient’s own IgG. RF will agglutinate IgG-coated latex beads.
Thermo Scientifics’ Opti-bind™ and Opti-link™ provide control sensitivity, specificity and stability. Various levels of carboxylic acid groups are provided for easy covalent coupling. They are packaged without surfactants, so they can be used directly from the bottle without pre-cleaning.

Brand: Thermo Scientific

  • Item no: 93101891020350
  • Product: Dyed and Fluorescent Particles
  • Particle Size: 0.85 µm
  • Concentration: 2 % Solids sld low acid
  • Composition: Polystyrene
  • Surface Modification: Carboxylate modified
  • Volume / Quantity: 100 mL
  • Color: BLUE
  • NIST Traceable: NO
  • (Key) application: Life science and diagnostic research
  • (Sub) application: Agglutination test development
  • Application L1: Diagnostic assay development
  • Application L2: Agglutination test
  • Brand: Thermo Scientific

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