Cyto-Cal™

1.00

Consist of a blank (non) bead, a one peak positive fluorescent beads and a bead with 6 different fluorescent instensities. All line excitation and overall emission in the visible spectrum.

SKU: 1fe76258c157 Category:

Description

The purpose of a coloured or fluorescently dyed particle is its visibility with the naked eye or by a detector. By adding a dye to the particle it reduces signal to noise due to the contrast between the particle and its environment. Applications for these particles are countless. By modifying the surface of the particles, antibodies or proteins can be conjugated to their surfaces, making them ideally suited for diagnostic assay development, for instance for lateral flow assay development. We offer coloured and fluorescent dyed microspheres from Thermo Scientific, VDo Biotech and Applied Microspheres.

Consist of a blank (non) bead, a one peak positive fluorescent beads and a bead with 6 different fluorescent instensities. All line excitation and overall emission in the visible spectrum.

Polymer microspheres are a major component in commercially available diagnostic tests. To facilitate binding proteins or antibodies to their surfaces, different surface chemistries are available, suitable for all commonly used conjugation protocols. Dyed microspheres are available for visual detection methods and fluorescently dyed microspheres for devices using fluorescence as a method of detection.

Flow Cytometry – Instrument calibration
Flow cytometry is an analytical technique that is used study and characterize cells. A flow cytometer counts the cells and employs lasers to analyze their fluorescence and light scatter properties as they flow in a precisely focused liquid stream.
Flow cytometers require accurate calibration of each of the data channels in order to provide reliable information for comparison of multiple analyses of cell populations. This calibration is accomplished using very uniform microspheres with known size and fluorescence properties. Cyto-Cal™ is designed to simultaneously monitor flow cytometer stability, give relative fluorescence estimations of labeled cells and provide a check on instrument sensitivity and performance with weakly fluorescent samples.

  • Item no: FC3MV-KIT
  • Product: Dyed and Fluorescent Particles
  • Particle Size: 3,6 µm
  • Concentration: 1,5 x 10E7 / mL
  • Composition: Polystyrene
  • Surface Modification: n/a
  • Volume / Quantity: 2 mL
  • Color: FLUOR
  • NIST Traceable: No
  • (Key) application: Life science and diagnostic research
  • (Sub) application: Flow Cytometry – Instrument calibration
    Flow cytometry is an analytical technique that is used study and characterize cells. A flow cytometer counts the cells and employs lasers to analyze their fluorescence and light scatter properties as they flow in a precisely focused liquid stream.
    Flow cytometers require accurate calibration of each of the data channels in order to provide reliable information for comparison of multiple analyses of cell populations. This calibration is accomplished using very uniform microspheres with known size and fluorescence properties. Cyto-Cal™ is designed to simultaneously monitor flow cytometer stability, give relative fluorescence estimations of labeled cells and provide a check on instrument sensitivity and performance with weakly fluorescent samples.
  • Application L1: Flow cytometry
  • Application L2: Instrument monitoring
  • Brand: Applied Microspheres

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