Cyto-Plex™

1.00

Multiplex array analysis is a powerful technique enabling the simultaneous detection and quantitation of multiple analytes in a single sample. Using color coded microspheres, these tests can be carried out at flow cytometers. Cyto-Plex microspheres provide up to ten levels of red fluorescent intensities for analysis of ten different analytes. The Cyto-Plex microsphere kits consist of uniform particles with fluorescence intensities that are completely separated from each other. The microspheres are all the same size and have high-density carboxylated surfaces, providing very low non-specific binding and a consistent, uniform surface for coupling of a wide variety of anitbodies, nucleic acids and other biomolecules. Cyto-Plex microspheres have maximum emission at 700 nm, and can be excited with either 488 nm or 633 nm. Emission can be collected in 650 – 670 nm detector. Since there is little or no emission in FL1 and FL2, probes utilizing either of these channels can be effectively used as reporters.

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Description

The purpose of a coloured or fluorescently dyed particle is its visibility with the naked eye or by a detector. By adding a dye to the particle it reduces signal to noise due to the contrast between the particle and its environment. Applications for these particles are countless. By modifying the surface of the particles, antibodies or proteins can be conjugated to their surfaces, making them ideally suited for diagnostic assay development, for instance for lateral flow assay development. We offer coloured and fluorescent dyed microspheres from Thermo Scientific, VDo Biotech and Applied Microspheres.

Multiplex array analysis is a powerful technique enabling the simultaneous detection and quantitation of multiple analytes in a single sample. Using color coded microspheres, these tests can be carried out at flow cytometers. Cyto-Plex microspheres provide up to ten levels of red fluorescent intensities for analysis of ten different analytes. The Cyto-Plex microsphere kits consist of uniform particles with fluorescence intensities that are completely separated from each other. The microspheres are all the same size and have high-density carboxylated surfaces, providing very low non-specific binding and a consistent, uniform surface for coupling of a wide variety of anitbodies, nucleic acids and other biomolecules. Cyto-Plex microspheres have maximum emission at 700 nm, and can be excited with either 488 nm or 633 nm. Emission can be collected in 650 – 670 nm detector. Since there is little or no emission in FL1 and FL2, probes utilizing either of these channels can be effectively used as reporters.

Polymer microspheres are a major component in commercially available diagnostic tests. To facilitate binding proteins or antibodies to their surfaces, different surface chemistries are available, suitable for all commonly used conjugation protocols. Dyed microspheres are available for visual detection methods and fluorescently dyed microspheres for devices using fluorescence as a method of detection.

Flow cytometry is an analytical technique that is used study and characterize cells. A flow cytometer counts the cells and employs lasers to analyze their fluorescence and light scatter properties as they flow in a precisely focused liquid stream.
Multiplex array analysis is a powerful technique utilizing flow cytometry which enables the simultaneous detection and quantitation of multiple analytes in a single sample. Using color coded microspheres, these tests can now be carried out using commonly available instrumentation. Cyto-Plex™ from Thermo Scientific provides up to ten levels of red fluorescent intensities for simultaneous analysis of ten different substances. The uniform 4 or 5 um microspheres have high density carboxylated surfaces, providing very low non-specific binding and a consistent, uniform surface for coupling a wide variety of antibodies, nucleic acids and other biomolecules.

  • Item no: FM5CR05
  • Product: Dyed and Fluorescent Particles
  • Particle Size: 5 µm
  • Concentration: 7.3 x 10E7 / mL
  • Composition: Polystyrene
  • Surface Modification: Carboxylate modified
  • Volume / Quantity: 1 mL
  • Color: FL-RED
  • NIST Traceable: NO
  • (Key) application: Life science and diagnostic research
  • (Sub) application: Flow Cytometry Multiplex test development
  • Application L1: Flow cytometry
  • Application L2: Multiplex assay
  • Brand: Thermo Scientific

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