For those who are working in medical diagnostic test development and bioscience, when labelling and detection of very low concentrations of substances is required, we offer microspheres for both passive adsorption and covalent coupling protocols. To accommodate the behaviour of different type of antibodies, we offer microspheres with various surface chemistry modifications for both coupling procedures.
Achieve maximum reactivity in many diagnostic applications with Thermo Scientific™ Opti-Bind™ Polystyrene Sulfate Particles for use in turbidimetric/nephelometric assays, slide agglutination assays, chemiluminescent assays and in clinical diagnostics and molecular biology applications. Particles can be used direct from the bottle, without any pre-cleaning.
Our production does not utilize common surfactants (like SDS, Tween™ 20, Triton™ X-100) that can interfere with protein binding to particle surfaces. Polystyrene surfaces are very hydrophobic and adsorb proteins almost instantaneously. Thermo’s proprietary anionic surfactant does not interfere with the binding of proteins or cause proteins to desorb from microparticle surfaces.
• Available in a wide range of diameters (from 0.1 μm to 2.5 μm)
• No pre-washing
• Tight size distribution—CV<2%
• Optimized colloidal stability
Polymer microspheres are a major component in commercially available diagnostic tests. To facilitate binding proteins or antibodies to their surfaces, different surface chemistries are available, suitable for all commonly used conjugation protocols. Dyed microspheres are available for visual detection methods and fluorescently dyed microspheres for devices using fluorescence as a method of detection.
In Clinical Chemistry, Turbidimetry, a particle based analysis method (the name being derived from turbidity), is the process of measuring the loss of intensity of transmitted light due to the scattering effect of particles suspended in it. Light is passed through a filter creating a light of known wavelength which is then passed through a cuvette containing a solution. In nephelometry, the intensity of the scattered light is measured, usually, but not necessarily, at right angles to the incident light beam.
- Item no: 81000997100390
- Product: Particles for Assay Development
- Particle Size: 0.4 µm
- Concentration: 10 % Solids
- Composition: Polystyrene
- Surface Modification: Sulphate
- Volume / Quantity: 100 mL
- Color: WHITE
- NIST Traceable: NO
- (Key) application: Life science and diagnostic research
- (Sub) application: Diagnostic assay development
- Application L1: Agglutination, Turbidimetry, Nephelometry test development
- Application L2: Agglutination test
- Brand: Thermo Scientific