We offer you different procedures and manuals developed by the Particle Technology
of Thermo Scientific which are directly related to particle usage.
Adsorption and Covalent Coupling Procedures
For usage in particle assay development plain polystyrene particles (sulphate), or particles with surface modifications like Carboxylate Modified, Amine modified require a coupling with antibodies, proteins, DNA, RNA etc.
This procedure describes the process of covalent coupling and its optimization steps. Prior to covalent coupling there will be always a partial passive adsorption to the beads.
Working with Particles
The following guidelines provide helpful tips on how to use our particles and should be followed accordingly. Please read the literature that accompanies your particle product for any special handling notes.
Sera-Mag Oligo(dT) coupling procedure
Thermo Scienti c Sera-Mag Oligo(dT)-coated magnetic particles (MG-OL) easily isolate and
extract valuable mRNA from a variety of sources for use in such applications as RT-PCR, cDNA library construction, cDNA microarrays, af nity puri cation, primer extension and subtractive hybridization.
Thermo Scienti c complete particle technology solutions provide simple protocols for working with particles and concrete data backed by 30 years of proprietary applications research in our labs.
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